Journal of Visualized Experiments

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2009

Issue 27: May

Issue 26: April

Issue 25: March

 

 

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What is JoVE?

Journal of Visualized Experiments (JoVE) is a video journal for biological research.

 

07/03/2009
Using the Horseshoe Crab, Limulus Polyphemus, in Vision Research
Jiahui S. Liu, Christopher L. Passaglia
Department of Biomedical Engineering, Boston University
In this video we perform electroretinogram recording, optic nerve recording, and intraretinal recording with the American horseshoe crab, Limulus Polyphemus. These electrophysiological paradigms can be used for investigating the neural basis of vision in a research or teaching lab.
07/02/2009
Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
Melanie Jungblut, Karen Oeltze, Irene Zehnter, Doris Hasselmann, Andreas Bosio
Miltenyi Biotec, GmbH
Dissociating cells from specific tissue types requires specific parameters for tissue agitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on lung tissue is explained.
07/01/2009
Aaron Ciechanover was born in Haifa, Israel in October 1947. Ciechanover shared the Nobel Prize in Chemistry in 2004 with Avram Hershko and Irwin Rose for their discovery of ubiquitin-mediated protein degradation.
06/30/2009
Making Gynogenetic Diploid Zebrafish by Early Pressure
Charline Walker1, Greg S. Walsh2, Cecilia Moens2
1Institute of Neuroscience, University of Oregon, 2Division of Basic Science, Fred Hutchinson Cancer Research Center
This is a method for generating gynogenetic diploid zebrafish embryos (embryos whose only genetic contribution comes from the mother) by blocking the second meiotic division immediately after fertilization with ultraviolet light-inactivated sperm. EP embryos are not fully homozygous due to recombination during the first meiotic division, however they are homozygous at all loci that have not been separated from their centromere by recombination.
06/29/2009
Focal Ca2+ Transient Detection in Smooth Muscle
John S. Young, Robert J. Amos, Keith L. Brain
Department of Pharmacology, University of Oxford
Details methods for high-resolution Ca2+ imaging of smooth muscle within isolated organs, including: preparation of the tissue, image acquisition and data analysis.

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About Jove

 

Journal of Visualized Experiments (JoVE) is an online research journal employing visualization to increase reproducibility and transparency in biological sciences.

 

ISSN 1940-087X

 

 

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